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Hassan, Mohammad Mahmudul
- Prevalence and Multidrug-Resistant Pattern of Salmonella from the Eggs and Egg-Storing Trays of Retail Markets of Bangladesh
Authors
1 Department of Physiology, Biochemistry and Pharmacology, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4225, BD
2 Department of Animal Science and Nutrition, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4225, IN
3 Department of Dairy and Poultry Science, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4225, BD
4 Department of Dairy and Poultry Science, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225, BD
5 EcoHealth Alliance, New York, NY, US
Source
International Journal of One Health, Vol 2 (2016), Pagination: 7-11Abstract
Aim: Salmonellosis is one of the most common and widely distributed foodborne illnesses in human, and multidrugresistance of Salmonella spp. has increased in developing countries with the indiscriminate use of antibiotics in the poultry production system. A cross-sectional study was conducted on randomly selected retail markets of Chittagong City Corporation to determine the prevalence and antimicrobial resistance pattern of Salmonella isolated from commercial layer eggs, eggshell surface, and egg-storing trays.
Materials and Methods: Chicken eggs, egg surface, and egg-storing trays samples from the retail markets were collected for isolating Salmonella spp. (bacteriological culture methods) followed by antimicrobial susceptibility testing (disc diffusion method) against Salmonella isolates during the period from July to December 2013.
Results: Out of the 310 layer eggs, egg surface, and egg-storing trays samples, the highest prevalence of Salmonella spp. was found in eggs trays (57.15%) and the lowest (13.33%) in eggs and the prevalence differed significantly (p<0.01). On the other hand, the prevalence was higher (45%) in samples of Pahartali bazar and lower (31.43%) in samples of Bohderhat bazar but the variation among the sites was not varied significantly (p>0.05). Isolated Salmonella was tested for resistance to eight different antimicrobial agents, using disc diffusion method. Among eight antimicrobial tested (n=111), 100% resistance were found to ampicillin and amoxicillin followed by erythromycin (60-100%), tetracycline (72-93%), ciprofloxacin (22-66%), colistin (27-66%), enrofloxacin (42-54%), and pefloxacin 23.07% across the study sites. Ciprofloxacin remained sensitive in 40.9% cases and, pefloxacin and colistin appeared to be almost sensitive (61-72%) against Salmonella isolates at studied areas. Salmonella isolates showed multidrug-resistance pattern up to five of the eight antimicrobials tested.
Conclusion: It can be said that the rational use of antibiotics needs to be adopted in commercial poultry farming system of Bangladesh to prevent the emergence of drug-resistance Salmonella to protect the public health consequences.
Keywords
Antimicrobial, Public Health, Prevalence, Resistance, Salmonella.- Prevalence and Multidrug Resistance Pattern of Salmonella Isolated from Resident Wild Birds of Bangladesh
Authors
1 Department of Anatomy and Histology, Chittagong Veterinary and Animal Sciences University, Chittagong-4225, BD
2 Department of Physiology, Biochemistry and Pharmacology, Chittagong Veterinary and Animal Sciences University, Chittagong-4225, BD
3 Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University, Chittagong-4225, BD
4 Department of Animal Science and Nutrition, Chittagong Veterinary and Animal Sciences University, Chittagong-4225, BD
5 EcoHealth Alliance, New York, NY, US
Source
International Journal of One Health, Vol 2 (2016), Pagination: 35-41Abstract
Aim: Salmonellosis is one of the most common zoonotic diseases, and the presence of antimicrobial resistant Salmonella in wild birds is global public health threat. Throughout the last decades, multidrug resistance of Salmonella spp. has increased, particularly in developing countries. Therefore, a cross-sectional study was conducted to investigate the prevalence of Salmonella spp. and antimicrobial resistance pattern against Salmonella spp. from two species of resident wild birds namely house crow (Corvus splendens) and Asian pied starling (Gracupica contra).
Materials and Methods: Samples were collected from cloacal swabs of house crows and Asian pied starling for isolating Salmonella spp. (bacteriological culture methods) followed by antimicrobial susceptibility testing (disk diffusion method) against Salmonella spp. isolates during March to December 2014.
Results: The prevalence of Salmonella in Asian pied starling and house crows were 67% and 65%, respectively. Within the category of samples from different species, the variation in prevalence was not varied significantly (p>0.05). Isolated Salmonella spp. was tested for resistance to six different antimicrobial agents. Among six antimicrobial tested, 100% resistance were found to penicillin, oxacillin, and clindamycin followed by erythromycin (50-93%), kanamycin (7-20%), and cephalothin (30-67%) from both species of birds. Kanamycin remained sensitive in (70-73%), cephalothin (26-70%), and erythromycin appeared to be (0-30%) sensitive against Salmonella spp. isolates. Isolated Salmonella spp. was multidrug resistant up to three of the six antimicrobials tested.
Conclusion: It can be said that the rational use of antimicrobials needs to be adopted in the treatment of disease for livestock, poultry, and human of Bangladesh to limit the emergence of drug resistance to Salmonella spp.
Keywords
Antimicrobial, Prevalence, Resistance, Resident Wild Birds, Salmonella.- Antimicrobial Residues in Tissues and Eggs of Laying Hens at Chittagong, Bangladesh
Authors
1 EcoHealth Alliance, New York, NY, US
2 Department of Physiology, Biochemistry and Pharmacology, Chittagong Veterinary and Animal Sciences University, Chittagong 4225, BD
3 Department of Anatomy and Histology, Chittagong Veterinary and Animal Sciences University, Chittagong 4225, BD
4 Bangladesh Council of Scientific and Industrial Research, Chittagong 4225, BD
5 Department of Anthropology, Jahangirnagar University, Savar, IN
6 Department of Animal Science and Nutrition, Chittagong Veterinary and Animal Sciences University, Chittagong 4225, BD
Source
International Journal of One Health, Vol 2 (2016), Pagination: 75-80Abstract
Aim: Antimicrobial residue in animal food products is an important index of food safety. Antimicrobial residues could result from chemotherapeutic or chemoprophylactic use of drugs in food animals. This occurrence of residue in animal food products has received enormous worldwide attention from some local, international, and public health agencies. A crosssectional study was conducted from July to December 2009 to detect the antibiotic residues in tissues and eggs of laying hens at Chittagong of Bangladesh.
Materials and Methods: Microbial inhibition test (MIT) and thin layer chromatography (TLC) methods were used to detect antibacterial residues in poultry tissues (liver, kidney, breast, and thigh muscles) and eggs. The bacteria and pH of the MIT method were as follows: Bacillus subtilis on test agar medium with a pH of 7.2, Bacillus cereus with a pH of 6.0, and Escherichia coli at pH with an 8.0.
Results: The overall prevalence of antibiotic residues detected by MIT was 64% in liver, 63% in kidney, 56% in breast muscle, 50% in thigh muscle, and 60% in eggs. There was significant variation in results between MIT and TLC (p<0.05). Tetracycline residues were found in 48% in liver, 24% in kidneys, 20% in thigh muscles, 26% in breast muscles, and 36% in eggs. Ciprofloxacin residues were found 46% in liver, 42% in kidneys, 34% in thigh muscles, 30% in breast muscles, and 30% in eggs. Enrofloxacin residues were found 40% in livers, 36% in kidneys, 24% in thigh muscles, 20% in breast muscles, and 26% in eggs. Amoxicillin residues were found 48% in livers, 30% in kidneys, 26% in thigh muscles, 22% in breast muscles, and 24% in eggs. The most frequently detected antibiotic residues by both MIT and TLC were found in liver tissue, tetracycline (48%), ciprofloxacin (46%), enrofloxacin (40%), and amoxicillin (42%) were found in liver. Breast muscle tissue was least likely to contain antibiotic residues (24%). Tetracycline (p=0.01) and amoxicillin (p=0.03) residues had significant variation among the various poultry tissues and eggs.
Conclusions: A high percentage of tissues and eggs that could be available for human consumption had antibiotic residues. This study suggests that poultry meat and eggs should not be circulated to markets until the end of the drug's withdrawal period. It is also recommended to observe the withdrawal period of drugs before poultry slaughter or table egg distribution to avoid antimicrobial resistance and to inform both owners and consumers about the risks of antibiotic residues.